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Ently used for encapsulating and successfully [39] delivering a variety of anticancerEntly used for encapsulating and successfully [39] delivering a variety of anticancer drugs (reviewed in ). Problems of stability, cytotoxicity and conservation may represent constrains that require further optimized [42] formulations .POLYMERIC NANOPARTICLESPolymer-based nanoparticle delivery systems (polym
Against Buffer B (25 mM HEPES-NaOH (pH 8.0), 1 mM EDTA, 1 mM dithiothreitol, 10 (v/v) glycerol, 100 mM NaCl) and applied to a HiPrep Sephacryl S-300 column (GE Healthcare). DnaK was concentrated and stored at 70 . DnaJ was purified from MC4100 cells harboring pKV1961 (pKV1142 trcp-dnaJ). The cells were grown to late log phase in L broth containing 50 g/ml ampicillin at 30 , and DnaJ synthesis w
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Taken into consideration , when using viral vectors for vaccination, including potentialTaken into consideration , when using viral vectors for vaccination, including potential integration, transcriptional activation of oncogenes, preexisting immunity against the viral vector and limitations , in transgenic capacity size. Several recombinant viral vectors, both RNA and DNA viruses, have been
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K authentic promoters were amplified byPCR and cloned under the trc promoter. In the case of DnaKHis and DnaJ-His, six histidine codons were added to the 3 -end of the corresponding genes on the pKV1142 derivatives. Both DnaK-His and DnaJ-His have two extra amino acids, arginine and serine, between the last amino acid residue of the intact protein and the His tag, which is derived from the BglII s
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N, however, when cells were exposed to heterologus antigens they produced significantly higher amounts of TNF in relation to IFN[41]. During primary infections in mice, dengue specific CD4+ cells were low; however, in all four viral serotypes of a secondary infection there is a marked increase CD4+ response. Not only did CD4+ cells increase IFN production, but they increased CD8+ effector cell act
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