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Immunohistochemistry on primary ovarian cancer cells, Patricia Ewing for pathological analysis of the ovarian cancer tumors, and Wendy Onstenk and Bianca Mostert for providing us with the ascites samples. The results shown here are in part based upon data generated by the TCGA Research Network: http://cancergenome.nih.gov/. This project was financially supported by a grant from the Dutch Cancer So
Ed. Tel.: 81-76-264-6229; Fax: 81-76-264-6230; E-mail: mkanemo@staff.kanazawa-u.ac.jp.The abbreviations used are: HSP, heat shock protein; Hsp70, heat shock protein 70; Hsp40, heat shock protein 40; IPTG, isopropyl- -D-thiogalactopyranoside; CBB, Coomassie Brilliant Blue.JUNE 1, 2012 ?VOLUME 287 ?NUMBERJOURNAL OF BIOLOGICAL CHEMISTRYChaperone Binding toone system effects on 32 degradation have nev
On cell number) was used for cytospin preparation. Slides were air-dried for 30 min, fixed O/N in 10 formalin, then stored in 70 ethanol at 4 , until IHC staining. IHC staining was performed using Ventana Benchmark ULTRA automated slide stainers, and antibodies against pan keratin (Neomarkers, Fremont,CA, USA, MS-343-P), EpCAM (DAKO, Heverlee, Belgium, M0804) and p53 (DAKO, M7001).Pathway ana
With the continuing increase in the value of electrical power from traditional sources, substitute means of energy era these kinds of as harnessing the power of the sunlight and wind are becoming the subject matter of near scrutiny. Wind electricity has ever more turn out to be the subject of extreme speculation by people who are concerned about growing power expenses and who are really involved a
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RpoDhs-lacZ)) (27), which is a MC4100 derivative that lacks the rpoH gene but is able to grow at 37 due to the overproduction of GroEL and GroES, was used for the purification of 32. AD202 (MC4100 ompT::kan) (28) was used for the purification of FtsH. HB101 (supE (mcrC-mrr) recA ara-14 proA lacY galK rpsL xyl-5 mtl-1 leuB thi-1) and MC4100 were used for the purification of DnaK and DnaJ, respect
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E presents with a multitude of surfaces which enables them to interact with a diverse set of enzymes, cell membranes, and other partners [120]. The overall question, however remains, as to why the IDPs are both associated with and required for biomineralization. It might be argued, that IDPs arose by geneAuthor Manuscript Author Manuscript Author Manuscript Author Manuscript(d) (c)Matrix Biol. Aut
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