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Ases its substrates. After dissociation from Hsp70, the polypeptide chains fold into a functional three-dimensional architecture. A model has been proposed in which Hsp40 first recognizes and binds substrate polypeptides and then transfers them to Hsp70. However, in some cases, DnaK itself recognizes and binds to substrate polypeptides independent of DnaJ, as in the case of 32 (24). Because most o
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Induced cell death poptosis, autophagy and senescence. Febs J. 2010;277(1):2?1. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell. 2009;136(2):215?3. van Jaarsveld MT, Helleman J, Berns EM, Wiemer EA. MicroRNAs in ovarian cancer biology and therapy resistance. Int J Biochem Cell Biol. 2010;42(8):1282?0. van Jaarsveld MT, Helleman J, Boersma AW, van Kuijk PF, van Ijcken WF, De
Epartment of Genetics, Erasmus University Medical Center, 3015 CN, Rotterdam, The Netherlands. 4 Present address: Max Planck Institute for Molecular Genetics, Berlin, Germany. Received: 13 April 2015 Accepted: 28 OctoberParts of the 3'UTR of CCND1, GRB2, ERK2, RSK1 and RSK2 (see Additional file 1: Figure S7) were PCR amplified from human genomic DNA (Promega) introducing a XhoI (5'-end) and a NotI
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Ray facility. JH, RM, JP, EB and JV contributed to the design of the experiments and interpretation of the data. EW conceived, designed and coordinated experiments, helped interpreting the data and drafting the manuscript. All authors have read and approved the manuscript. Acknowledgements The authors are grateful to Dr. Seiji Nato (Kyushu University, Fukuoka, Japan) and to Dr. Kevin Scanlon (Keck
Mmunity; difficult to levels of antigens inside target cells; manufacture andMmunity; difficult to levels of antigens inside target cells; manufacture and store sustained gene expression; vector itself can provide an adjuvant effect Nano-scale size Ability to induce humoral and Challenges in B-cell, CD4+ and materials made of cellular immune responses; increased vaccine formulation, cytotoxi
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Immunohistochemistry on primary ovarian cancer cells, Patricia Ewing for pathological analysis of the ovarian cancer tumors, and Wendy Onstenk and Bianca Mostert for providing us with the ascites samples. The results shown here are in part based upon data generated by the TCGA Research Network: http://cancergenome.nih.gov/. This project was financially supported by a grant from the Dutch Cancer So
Ed. Tel.: 81-76-264-6229; Fax: 81-76-264-6230; E-mail: mkanemo@staff.kanazawa-u.ac.jp.The abbreviations used are: HSP, heat shock protein; Hsp70, heat shock protein 70; Hsp40, heat shock protein 40; IPTG, isopropyl- -D-thiogalactopyranoside; CBB, Coomassie Brilliant Blue.JUNE 1, 2012 ?VOLUME 287 ?NUMBERJOURNAL OF BIOLOGICAL CHEMISTRYChaperone Binding toone system effects on 32 degradation have nev
On cell number) was used for cytospin preparation. Slides were air-dried for 30 min, fixed O/N in 10 formalin, then stored in 70 ethanol at 4 , until IHC staining. IHC staining was performed using Ventana Benchmark ULTRA automated slide stainers, and antibodies against pan keratin (Neomarkers, Fremont,CA, USA, MS-343-P), EpCAM (DAKO, Heverlee, Belgium, M0804) and p53 (DAKO, M7001).Pathway ana
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